| 1. | Quality analysis of chinese mitten crab eriocheir sinensis in yangchenghu lake 阳澄湖中华绒螯蟹品质分析 |
| 2. | Effects of embryonic developmental stages in vitro from fertilized eggs in chinese mitten crab eriocheir sinensis on larva rearing 不同发育期中华绒螯蟹胚胎离体孵化和幼体培育的研究 |
| 3. | Zoobenthos were affected by mitten crabs in two ways : direct feeding on benthos and indirect effect through damage of submerged macrophytes 河吸对底栖动物的影响一是直接觅食,二是通过破坏水草间接影响。 |
| 4. | It was appropriate for cloning the full - length cdnas of the genes related to the ovary development of the mitten crab Northern印迹分析结果表明ejoi基因在m期卵巢高表达。 ejoi基因的genbank检索号为: ay185917 。 |
| 5. | Sinensis might be recognized . the mitten crabs and freshwater crabs are different lineages of nonmarine crabs . the former belongs to adults - limnic , the latter to entirely inland forms 绒螯蟹与淡水蟹是2个不同类型的非海洋蟹类,前者属于成体湖沼型;后者属于完全内陆型。 |
| 6. | The phylogenetic relationship of the mitten crab and other grapsoid crabs suggested that eriocheir is monophyletic , and the estimated divergence time of the mitten crabs with its marine varunid ancestor is about 8 . 9 ~ 10 . 9 mya 淡水蟹类的系统发生研究显示,华溪蟹属与仿溪蟹属起源于一个共同的海生蟹类祖先,二者之间的歧异时间在36 44mya 。 |
| 7. | Chapter 3 sequencing cdna fragments of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) partial clones were sequenced which were selected from the differentially expressed clones screened by cdna macroarray analysis 3中华绒螯蟹卵巢发育相关基因cdna片段的序列测定从cdna微阵列筛选的差异表达克隆中选取部分克隆进行测序。 |
| 8. | Rearrangements have been occurred in at least twelve mitochondrial genes , which have drastically altered the gene order of the mitten crab e . japonica sinensis to the putative ancestral pancrustacean ( crustacean / insecta ) gene order 中华绒螯蟹线粒体基因组与推测的泛甲壳类原始的线粒体基因顺序相比,至少有12个基因发生了重排,造成线粒体基因排列顺序显著的变化。 |
| 9. | Two - directional ( forward and backward ) ssh was performed on cdnas of the mitten crab ovaries which were either at stage ii or at stage iii . two - directional subtracted cdna fragments were cloned into pinpoint plasmid vectors , and the vectors were transformed into e . coli jm109 其中以期卵巢为试验方( tester ) ,期卵巢为驱动方( driver )进行正向差减杂交;以期卵巢为试验方,期卵巢为驱动方进行反向差减杂交。 |
| 10. | Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method , then the inserted fragments in plasmids were amplified by pcr 2用cdna微阵列筛选中华绒螯蟹卵巢发育相关基因将差减cdna文库中的所有克隆用碱裂解法提取质粒,然后以提取的质粒为模板,用pcr方法扩增插入片段。 |